Real Time Pcr Graph. In real time pcr we use both types of graph to examine the data. Real time pcr can be used quantitatively.
Real time pcr components 5 1 4 real time pcr analysis terminology 7 1 5 real time pcr fluorescence detection systems 11 1 6 melting curve analysis 15 1 7 use of passive reference dyes 16 1 8 contamination prevention 17 1 9 multiplex real time pcr 17 1 10 internal controls and reference genes 19 1 11 real time pcr instrument calibration 20. Please login to order a test. A real time polymerase chain reaction real time pcr also known as quantitative polymerase chain reaction qpcr is a laboratory technique of molecular biology based on the polymerase chain reaction pcr.
Note that there is a straight line relationship between the amount of dna and cycle number when you look on a logarithmic scale.
Rn is the fluorescence of the reporter dye divided by the fluorescence of a passive reference dye. A in this view rn is plotted against pcr cycle number. This is because pcr amplification is a exponential reaction. Real time pcr can be used quantitatively.
